3/23/2023 0 Comments Step seq cloneThe cells are ruptured, and DNA is separated into single strands-all on the membrane. Then a porous membrane is laid over the surface of each plate, and cells adhere to the membrane. Bacterial colonies constituting a library are grown in a collection of Petri dishes. For example, if a mouse gene has already been cloned, then that clone can be used to find the equivalent human clone from a human genomic library. Most commonly, a cloned DNA segment that shows homology to the sought gene is used as a probe. There are several ways of finding it, depending on the specific gene concerned. If the library encompasses the whole genome of an organism, then somewhere within that library will be the desired clone. The next step after cloning, therefore, is to find and isolate that clone among other members of the library. In general, cloning is undertaken in order to obtain the clone of one particular gene or DNA sequence of interest. SpaceNext50 Britannica presents SpaceNext50, From the race to the Moon to space stewardship, we explore a wide range of subjects that feed our curiosity about space!.Learn about the major environmental problems facing our planet and what can be done about them!
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